Genetic analysis and fine-mapping of a dwarfing with withered leaf-tip mutant in rice

Liang Jiang; Longbiao Guo; Hua Jiang; Dali Zeng; Jiang Hu; Liwen Wu; Jian Liu; Zhenyu Gao; Qian Qian

doi:10.1016/S1673-8527(08)60226-X

Volume 35 Issue 12

Dec. 2008

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Article Navigation > Journal of Genetics and Genomics > 2008 > 35(12): 715-721

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Genetic analysis and fine-mapping of a dwarfing with withered leaf-tip mutant in rice

doi: 10.1016/S1673-8527(08)60226-X

a
State Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
b
Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China

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Corresponding author: E-mail address: guolongb@mail.hz.zj.cn (Longbiao Guo); E-mail address: qianqian188@hotmail.com (Qian Qian)
Received Date: 2008-07-22
Accepted Date: 2008-10-06
Rev Recd Date: 2008-09-28

Available Online: 2008-12-23

Publish Date: 2008-12-20

Abstract

Abstract

A dwarf mutant of rice (Oryza sativa L.) by mutagenesis of ethylene methylsulfonate (EMS) treatment from Nipponbare was identified. The mutant exhibited phenotypes of dwarfism and withered leaf tip (dwl1). Based on the internode length of dwl1, this mutant belongs to the dm type of dwarfing. Analysis of elongation of the second sheath and α-amylase activity in endosperm showed that the phenotype caused by dwl1 was insensitive to gibberellin acid treatment. Using a large F₂ population derived from a cross between the dwl1 and an indica rice variety, TN1, the DWL1 gene was mapped to the terminal region of the long arm of chromosome 3. Fine-mapping delimited it into a 46 kb physical distance between two STS markers, HL921 and HL944, where 6 open reading frames were predicted. Cloning of DWL1 will contribute to dissecting molecular mechanism that regulates plant height in rice, which will be beneficial to molecular assisted selection of this important trait.
- mutant,
- dwarf,
- fine mapping,
- rice

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References(44)

References

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