The Altered Responses of a New Mutant long life span 1 to Cytokinin in Arabidopsis thaliana

Xinghua Shao; Ru Yang; Jingyu Wang; Qi Fang; Bin Yao; Yang Wang; Yue Sun; Xiaofang Li

doi:10.1016/j.jgg.2011.10.005

Volume 39 Issue 3

Mar. 2012

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Article Navigation > Journal of Genetics and Genomics > 2012 > 39(3): 139-148

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The Altered Responses of a New Mutant long life span 1 to Cytokinin in Arabidopsis thaliana

doi: 10.1016/j.jgg.2011.10.005

School of Life Science, East China Normal University, Shanghai 200062, China

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Corresponding author: E-mail address: xfli@bio.ecnu.edu.cn (Xiaofang Li)
Received Date: 2011-07-15
Accepted Date: 2011-10-26
Rev Recd Date: 2011-10-25

Available Online: 2011-12-20

Publish Date: 2012-03-20

Abstract

Abstract

Cytokinins are a class of essential plant hormones regulating plant growth and development. Although the two-component phosphorelay pathway of cytokinin has been well characterized, the intact cytokinin responses regulation picture still needs to be fully depicted. Here we report a new mutant, long life span 1 (lls1), which displays dwarf stature, curled leaves, numerous axillary branches and nearly 5-month life span. Exogenous cytokinin could not recover the phenotypes of the mutant. Moreover, mutation in lls1 suppressed the cytokinin-responsive phenotypes, including root and hypocotyl growth inhibition, anthocyanin accumulation, metaxylem promotion in primary root development. The induction of cytokinin-responsive genes, ARR5, AHP5, and CKX3, was also suppressed in lls1. According to quantitative RT-PCR (qRT-PCR) and microarray results, the basal expression of positive factors AHP5, ARR1, and ARR10 were down-regulated, while the negative factors ARR4 and ARR5 were up-regulated. Our results suggested that LLS1 gene might be involved in the regulation of cytokinin signaling. It was mapped to chromosome 4 where no other cytokinin relevant gene has been reported.
- ARRs,
- Cytokinin signaling

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References(40)

References

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