A non-canonical bipartite NLS dictates nuclear import and function of the <i>Drosophila</i> Pc protein

Tao He; Yu Fan; Yongchang Zeng; Jie Zhou; Renjie Jiao; Alan Jian Zhu; Min Liu

doi:10.1016/j.jgg.2025.12.003

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A non-canonical bipartite NLS dictates nuclear import and function of the Drosophila Pc protein

doi: 10.1016/j.jgg.2025.12.003

1. Sino-French Hoffmann Institute, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, Guangdong 511436, China;
2. State Key Laboratory of Membrane Biology, School of Life Sciences, Peking University, Beijing 100871, China;
3. Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China;
4. Peking University Chengdu Academy for Advanced Interdisciplinary Biotechnologies, Chengdu, Sichuan 610213, China

Funds:

We thank Dr. Lei Zhang, DSHB, Bloomington Drosophila Stock Center, Tsinghua Fly Center for fly stocks and reagents. We also thank Dr. Chunyan Shan at the National Center for Protein Science at Peking University for assistance with microscopic imaging. This work was supported by National Key Research and Development Program of China (2021YFA0805800 to A.J.Z. and M.L.), National Natural Science Foundation of China (32330026 to A.J.Z., 32170716 to M.L. and 32500731 to T.H.), State Key Laboratory of Membrane Biology (to A.J.Z.), the Peking-Tsinghua Center for Life Sciences (to A.J.Z. and M.L.). Y.F. was supported by a Boya Postdoctoral Fellowship.

Received Date: 2025-09-05
Accepted Date: 2025-12-08
Rev Recd Date: 2025-12-08

Available Online: 2025-12-16

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